![]() During the functional analysis of tTPs, we uncovered the minimum 10 amino acid sequence is sufficient for thylakoid lumen transport. tabacum transit peptides (NtTPs) and the functions of these peptides are confirmed with TP-GFP fusion proteins under fluorescence microscopy and chloroplast fractionation, followed by Western blot analysis. Typical chloroplast stroma-transit peptides and thylakoid lumen transit peptides (tTPs) are found in N. Four chloroplast thylakoid lumen TPs were isolated from Nicotiana tabacum and were functionally analyzed as thylakoid lumen TPs. This transport is facilitated by chloroplast transit peptides (TPs). Numerous proteins must be transported from the cytosol to chloroplasts to support photosynthesis. Chloroplasts are intracellular organelles responsible for photosynthesis to produce organic carbon for all organisms. ![]() ![]() This ten amino acids do not contain twin-arginine, which is required for typical chloroplast lumen translocation. The thylakoid transit peptide of tobacco oxygen-evolving enhancer protein contains a minimal ten amino acid sequences for thylakoid lumen transports. ![]()
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